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rabbit polyclonal anti glua2  (Millipore)


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    Millipore rabbit polyclonal anti glua2
    (A–D) <t>GLUA2</t> protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .
    Rabbit Polyclonal Anti Glua2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+glua2/glua2+antibody/pmc12013928-3-0-5
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti glua2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Astrocyte glypican 5 regulates synapse maturation and stabilization"

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.115374

    (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .
    Figure Legend Snippet: (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

    Techniques Used: Immunostaining

    (A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .
    Figure Legend Snippet: (A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .

    Techniques Used: Membrane, Marker, Immunostaining

    (A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .
    Figure Legend Snippet: (A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .

    Techniques Used: Immunostaining

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Modification, Saline, RNAscope, Multiplex Assay, Electron Microscopy, Negative Control, Software, Cell Culture, Fluorescence, Microscopy, Hybridization

    Related Articles

    Immunostaining:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Membrane:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Marker:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Recombinant:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Modification:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Saline:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    RNAscope:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Multiplex Assay:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Electron Microscopy:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Negative Control:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Software:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Cell Culture:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Fluorescence:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Microscopy:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025

    Hybridization:

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-S100b Abcam CAT# ab52642; RRID:AB_882426 Rabbit polyclonal anti GluA1 Millipore CAT# AB1504; RRID:AB_2113602 Rabbit polyclonal anti GluA2 Millipore CAT# AB1768-I; RRID:AB_2313802 Guinea pig polyclonal anti VGlut1 Millipore CAT# AB5905; RRID:AB_2301751 Guinea pig polyclonal anti VGlut2 Millipore CAT# AB2251; RRID:AB_2665454 Rat anti HA tag Roche CAT# 11867423001; RRID:AB_390918 Rabbit anti Glypican 5 Thermo Fisher CAT# PA5-83490 RRID:AB_2790643 Mouse anti PSD95 Thermo Fisher CAT# MA1-045 RRID:AB_325399 Rabbit anti VGlut2 Synaptic Systems CAT# 135-403 RRID:AB_887883 Goat anti Rabbit Alexa 488 Molecular probes CAT# A11034; RRID:AB_2576217 Goat anti Rabbit Alexa 594 Molecular probes CAT# A11037; RRID:AB_2534095 Goat anti Guinea pig Alexa 594 Molecular probes CAT# A11076; RRID:AB_141930 Goat anti Rat Alexa 488 Molecular probes CAT# A11006; RRID:AB_141373 Goat anti Mouse Alexa 488 Molecular probes CAT# A11029; RRID:AB_138404 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher CAT# 11960044 Neurobasal Thermo Fisher CAT# 21103049 Penicillin-Streptomycin Thermo Fisher CAT# 15140-122 Glutamax Thermo Fisher CAT# 35050-061 Sodium pyruvate Thermo Fisher CAT# 11360-070 FBS Thermo Fisher CAT# 10437028 N-acetyl-L-cysteine Sigma CAT# A8199 Insulin Sigma CAT# I1882 Triiodo-thyronine Sigma CAT# T6397 Transferrin Sigma CAT# T1147 Forskolin Sigma CAT# F6886 BSA Sigma CAT# A4161 Progesterone Sigma CAT# P6149 Putrescine Sigma CAT# P5780 Sodium selenite Sigma CAT# S9133 B27 Thermo Fisher CAT# 17504044 BDNF Peprotech CAT# 450-02 CNTF Peprotech CAT# 450-13 Hydrocortisone Sigma CAT# H0888 Recombinant mouse glypican 5 R&D CAT# 2689-G5-050/CF Dulbeco’s modified phosphate buffered saline (DPBS) HyClone CAT# SH30264 16% PFA solution EMS CAT# 50980487 PFA powder EMS CAT# 19208 L-lysine Sigma CAT# L5501 Goat serum Thermo Fisher CAT# 16210072 SlowFade Gold with DAPI mounting media Thermo Fisher CAT# S36939 (Continued on next page) 18 Cell Reports 44, 115374, March 25, 2025



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    (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

    Journal: Cell reports

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

    doi: 10.1016/j.celrep.2025.115374

    Figure Lengend Snippet: (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Techniques: Immunostaining

    (A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .

    Journal: Cell reports

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

    doi: 10.1016/j.celrep.2025.115374

    Figure Lengend Snippet: (A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .

    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Techniques: Membrane, Marker, Immunostaining

    (A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .

    Journal: Cell reports

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

    doi: 10.1016/j.celrep.2025.115374

    Figure Lengend Snippet: (A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .

    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Techniques: Immunostaining

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

    doi: 10.1016/j.celrep.2025.115374

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit polyclonal anti GluA2 , Millipore , CAT# AB1768-I; RRID:AB_2313802.

    Techniques: Recombinant, Modification, Saline, RNAscope, Multiplex Assay, Electron Microscopy, Negative Control, Software, Cell Culture, Fluorescence, Microscopy, Hybridization

    Journal: iScience

    Article Title: Disuse plasticity limits spinal cord injury recovery

    doi: 10.1016/j.isci.2025.112180

    Figure Lengend Snippet:

    Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

    Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy

    Journal: iScience

    Article Title: Disuse plasticity limits spinal cord injury recovery

    doi: 10.1016/j.isci.2025.112180

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

    Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy